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stat2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc stat2
    Stat2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+stat2/Stat2+Rabbit+mAb/pmc13039202-279-64-80
    Average 96 stars, based on 247 article reviews
    stat2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Tubule‐Derived IFN‐α Promotes GSDMD‐Mediated Macrophage Pyroptosis to Drive Renal Inflammation and Fibrosis Through JAK2/STAT2 Activation
    Article Snippet: Ifn‐β (QM03294S), Ifn‐λ2 (QM08870S), and Ifnlr1 (QM08662S) primers were purchased from Beyotime (Shanghai, China).

    Article Title: Tubule-Derived IFN-α Promotes GSDMD-Mediated Macrophage Pyroptosis to Drive Renal Inflammation and Fibrosis Through JAK2/STAT2 Activation.
    Article Snippet: Validated constructs were transfected into 293T using Lipofectamine 3000 (L3000015, Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Western Blot:

    Article Title: Targeting KRAS Inhibitor–Resistant Pancreatic Cancer with an MUC1-C Antibody–Drug Conjugate
    Article Snippet: .. Immunoblotting was performed as described ( ) on total lysates from nonconfluent cells with anti-M1C [16564, 1:1,000 dilution; Cell Signaling Technology (CST), RRID: AB_2798765], anti–β-actin (A5441, 1:5,000 dilution; Sigma-Aldrich, RRID:AB_476744), anti–NF-κB p65 (8242, 1:2,000 dilution; CST, RRID: AB_10859369), anti-ALPPL2 (sc-134255, 1:500; Santa Cruz Biotechnology, RRID: AB_2258207), anti-STAT1 (9172, 1:1,000 dilution; CST, RRID: AB_2198300), anti-ERK (9107, 1:1,000; CST, RRID: AB_10695739), anti–p-ERK (4377, 1:1,000; CST, RRID: AB_331775), anti-MRAS (PA5-112613, 1:1,000 dilution; Thermo Fisher Scientific, RRID: AB_2867348), anti-SHOC2 (53600, 1:1,000; CST, RRID: AB_2799440), anti-PP1 (sc-7482, 1:1,000; Santa Cruz Biotechnology, RRID: AB_628177), anti–p-STAT1 (9171, 1:1,000 dilution; CST, RRID: AB_331591), anti-STAT2 (72604, 1:1,000 dilution; CST, RRID: AB_2799824), and anti-IRF9 (702322, 1:500 dilution; Thermo Fisher Scientific, RRID: AB_2633083). .. Subcellular fractions were analyzed by immunoblotting with anti–α-tubulin (2144, 1:1,000 dilution; CST, RRID: AB_2210548), anti-VDAC (ab15895, 1:1,000 dilution; Abcam, RRID: AB_2214787), anti–lamin B1 (66095-1-Ig, 1:1,000 dilution; Proteintech, RRID: AB_11232208), and anti-Histone H3 (ab1791, 1:5,000 dilution; Abcam, RRID: AB_302613).

    Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo
    Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, anti-STAT1, anti-p-STAT2, anti-STAT2, anti-RIG-I, anti-TLR-3, and anti-β-actin (Cell Signaling Technology, Danvers, MA), overnight at 4 0 C. The membranes were washed and incubated with the respective HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 1 h and developed using the ECLTM Prime Western Blotting System kit (GE Healthcare, Boston, MA). .. The ratio between phosphorylated and nonphosphorylated proteins, as well as the ratio of total protein expression (sum of phosphorylated and nonphosphorylated) and β-actin, were calculated using ImageJ software.

    Article Title: Crimean-Congo hemorrhagic fever virus NSm protein inhibits the type I interferon signaling by binding to STAT2.
    Article Snippet: .. Western blotting was performed as previously described using the following antibodies [10,12,13]: anti-HA (clone 3F10; Roche), anti-His (clone 9F2; Fujifilm Wako), anti-FLAG (catalog no. PM020; MBL), anti-VSIV M (clone 23H12; Kerafast), anti-VSIV G (clone P5D4; Sigma-Aldrich), anti-STAT2 (catalog no.: D9J7L; Cell Signaling Technology), anti-STAT1 (catalog no.: D19KY; Cell Signaling Technology), anti-STAT2 (phosphor Y690) (catalog no.: D3P2P; Cell Signaling Technology), anti-STAT1 (phosphor Y701) (catalog no.: D4A7; Cell Signaling Technology), anti-CCHFV Gn (in-house production) or anti-β-actin (catalog no. AC-15; Sigma-Aldrich). .. The band intensities of VSIV-G, VSIV-M, STAT1, pSTAT1, STAT2, pSTAT2, NSm-FLAG, and β-actin were quantified using ImageJ software (National Institutes of Health).

    Article Title: Crimean-Congo hemorrhagic fever virus NSm protein inhibits the type I interferon signaling by binding to STAT2
    Article Snippet: .. Western blotting was performed as previously described using the following antibodies [ , , ]: anti-HA (clone 3F10; Roche), anti-His (clone 9F2; Fujifilm Wako), anti-FLAG (catalog no. PM020; MBL), anti-VSIV M (clone 23H12; Kerafast), anti-VSIV G (clone P5D4; Sigma-Aldrich), anti-STAT2 (catalog no.: D9J7L; Cell Signaling Technology), anti-STAT1 (catalog no.: D19KY; Cell Signaling Technology), anti-STAT2 (phosphor Y690) (catalog no.: D3P2P; Cell Signaling Technology), anti-STAT1 (phosphor Y701) (catalog no.: D4A7; Cell Signaling Technology), anti-CCHFV Gn (in-house production) or anti-β-actin (catalog no. AC-15; Sigma-Aldrich). .. The band intensities of VSIV-G, VSIV-M, STAT1, pSTAT1, STAT2, pSTAT2, NSm-FLAG, and β-actin were quantified using ImageJ software (National Institutes of Health).

    Incubation:

    Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo
    Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, anti-STAT1, anti-p-STAT2, anti-STAT2, anti-RIG-I, anti-TLR-3, and anti-β-actin (Cell Signaling Technology, Danvers, MA), overnight at 4 0 C. The membranes were washed and incubated with the respective HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 1 h and developed using the ECLTM Prime Western Blotting System kit (GE Healthcare, Boston, MA). .. The ratio between phosphorylated and nonphosphorylated proteins, as well as the ratio of total protein expression (sum of phosphorylated and nonphosphorylated) and β-actin, were calculated using ImageJ software.

    Article Title: Distinct ZIKV strain signatures and type I IFN modulation reveal a protective role of brain endothelial interferon signaling in vitro and in vivo
    Article Snippet: The samples were subjected to a 10% polyacrylamide gel electrophoresis (SDS-PAGE), followed by transfer to a nitrocellulose membrane (Merck). .. The membranes were blocked with 10% TBS containing 5% bovine serum albumin (BSA; Merck) for 1 h, and incubated with the antibodies anti-p-STAT1, antiSTAT1, anti-p-STAT2, anti-STAT2, anti-RIG-I, anti-TLR-3, and anti-b-actin (Cell Signaling Technology, Danvers, MA), overnight at 40C. .. The membranes were washed and incubated with the respective HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories Inc., West Grove, PA) for 1 h and developed using the ECLTM Prime Western Blotting System kit (GE Healthcare, Boston, MA).



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    Generation of conditional <t>Stat2</t> KO mice. ( a ) Targeting strategy used to construct Stat2 fl/fl mice with exons 5 through 8 flanked with loxP sites. E represents exons with their corresponding number. Brown boxes depict exons in wild type allele and green boxes depict exons after successful integration of targeting vector. ( b ) Confirmation of loxP sites integration in the Stat2 gene by Southern blot analysis (9.8 kb DNA fragment). ( c ) Genotyping of mouse-tail DNA by PCR confirms deletion of Stat2 floxed allele after breeding with CMV-Cre mouse.
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    Image Search Results


    Generation of conditional Stat2 KO mice. ( a ) Targeting strategy used to construct Stat2 fl/fl mice with exons 5 through 8 flanked with loxP sites. E represents exons with their corresponding number. Brown boxes depict exons in wild type allele and green boxes depict exons after successful integration of targeting vector. ( b ) Confirmation of loxP sites integration in the Stat2 gene by Southern blot analysis (9.8 kb DNA fragment). ( c ) Genotyping of mouse-tail DNA by PCR confirms deletion of Stat2 floxed allele after breeding with CMV-Cre mouse.

    Journal: Immuno

    Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

    doi: 10.3390/immuno6010007

    Figure Lengend Snippet: Generation of conditional Stat2 KO mice. ( a ) Targeting strategy used to construct Stat2 fl/fl mice with exons 5 through 8 flanked with loxP sites. E represents exons with their corresponding number. Brown boxes depict exons in wild type allele and green boxes depict exons after successful integration of targeting vector. ( b ) Confirmation of loxP sites integration in the Stat2 gene by Southern blot analysis (9.8 kb DNA fragment). ( c ) Genotyping of mouse-tail DNA by PCR confirms deletion of Stat2 floxed allele after breeding with CMV-Cre mouse.

    Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

    Techniques: Construct, Plasmid Preparation, Southern Blot

    Impaired IFN-I signaling after conditional deletion of Stat2 by CMV-Cre—mediated recombination. ( a ) Loss of Stat2 gene expression validated in four different organs by qRT-PCR analysis. ( b ) Western blot analyses performed on several tissues confirm global Stat2 deletion ( Stat2 Δ / Δ ) by ubiquitous CMV-Cre recombinase. ( c ) Splenocytes of the indicated genotypes were left untreated or treated with IFN-β for 6 h, and ISG expression was determined by qRT-PCR. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Data are presented as SEM of three independent experiments. n.s; not significant.

    Journal: Immuno

    Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

    doi: 10.3390/immuno6010007

    Figure Lengend Snippet: Impaired IFN-I signaling after conditional deletion of Stat2 by CMV-Cre—mediated recombination. ( a ) Loss of Stat2 gene expression validated in four different organs by qRT-PCR analysis. ( b ) Western blot analyses performed on several tissues confirm global Stat2 deletion ( Stat2 Δ / Δ ) by ubiquitous CMV-Cre recombinase. ( c ) Splenocytes of the indicated genotypes were left untreated or treated with IFN-β for 6 h, and ISG expression was determined by qRT-PCR. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Data are presented as SEM of three independent experiments. n.s; not significant.

    Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

    Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing

    Stat2 Δ / Δ mice show enhanced tumor growth. ( a ) B16-F1 or ( b ) EL4 tumor cells were injected subcutaneously in WT, Stat2KO , Stat2fl/fl and Stat2 Δ/Δ . Values are shown as mean tumor volume determined over 20 days. Representative images of individual tumors are shown. *, p ≤ 0.05; **, p ≤ 0.01; and ***, p ≤ 0.001. n = 6–8 animals/group.

    Journal: Immuno

    Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

    doi: 10.3390/immuno6010007

    Figure Lengend Snippet: Stat2 Δ / Δ mice show enhanced tumor growth. ( a ) B16-F1 or ( b ) EL4 tumor cells were injected subcutaneously in WT, Stat2KO , Stat2fl/fl and Stat2 Δ/Δ . Values are shown as mean tumor volume determined over 20 days. Representative images of individual tumors are shown. *, p ≤ 0.05; **, p ≤ 0.01; and ***, p ≤ 0.001. n = 6–8 animals/group.

    Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

    Techniques: Injection

    Stat2 Δ / Δ fibroblasts have impaired antiviral response to type I IFNs. ( a ) Lung fibroblasts derived from the indicated genotypes were pretreated with IFN-β for 24 h. Cells were then infected with VSV-GFP (green) at an MOI of 0.01 and imaged after 24 h. Representative bright field and fluorescent images are shown. ( b ) Western blot analysis shows STAT2 expression in fibroblasts of indicated genotypes. Representative images are shown of n = 2.

    Journal: Immuno

    Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

    doi: 10.3390/immuno6010007

    Figure Lengend Snippet: Stat2 Δ / Δ fibroblasts have impaired antiviral response to type I IFNs. ( a ) Lung fibroblasts derived from the indicated genotypes were pretreated with IFN-β for 24 h. Cells were then infected with VSV-GFP (green) at an MOI of 0.01 and imaged after 24 h. Representative bright field and fluorescent images are shown. ( b ) Western blot analysis shows STAT2 expression in fibroblasts of indicated genotypes. Representative images are shown of n = 2.

    Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

    Techniques: Derivative Assay, Infection, Western Blot, Expressing

    Efficient targeted deletion of Stat2 in mouse tissues. Expression of STAT2 and STAT1 was analyzed in various tissues by Western blot analysis. ( a ) Bone marrow–derived cDCs generated from Stat2 fl/fl mice crossed with CD11c-Cre mice ( Stat2 Δ-cDC ). ( b ) Colons from Stat2 fl/fl mice crossed with Cdx2-Cre mice ( Stat2 Δ-CE ). ( c ) Lungs from Stat2 Δ-cDC and Stat2 Δ-CE mice were included for specificity of targeted deletion. Wild-type (WT) and Stat2KO mice served as positive and negative controls, respectively. ACTIN was used as an internal protein loading control.

    Journal: Immuno

    Article Title: Conditional Stat2 Knockout Mice as a Platform for Modeling Human Diseases

    doi: 10.3390/immuno6010007

    Figure Lengend Snippet: Efficient targeted deletion of Stat2 in mouse tissues. Expression of STAT2 and STAT1 was analyzed in various tissues by Western blot analysis. ( a ) Bone marrow–derived cDCs generated from Stat2 fl/fl mice crossed with CD11c-Cre mice ( Stat2 Δ-cDC ). ( b ) Colons from Stat2 fl/fl mice crossed with Cdx2-Cre mice ( Stat2 Δ-CE ). ( c ) Lungs from Stat2 Δ-cDC and Stat2 Δ-CE mice were included for specificity of targeted deletion. Wild-type (WT) and Stat2KO mice served as positive and negative controls, respectively. ACTIN was used as an internal protein loading control.

    Article Snippet: Antibodies against STAT1 (Cat#10144–2-AP), STAT2 (cat#51075–2-AP), β-Actin (cat#66009–1-Ig), HRP-conjugated anti-mouse IgG (cat#SA00001–1) and anti-rabbit-IgG (cat#SA00001–2) were purchased from Proteintech, Rosemont, IL, USA.

    Techniques: Expressing, Western Blot, Derivative Assay, Generated, Control